ClearLight (Hive Bee)
05-22-02 19:49
No 312786
      Electrophoresis separation  Bookmark   

  I had not normally thought of this as a viable purification method, however I just picked up copy of CL Stong's Amateur Scientist book from the 70's and there on the Electroph. article is a discussion about a 30 inch vertical plate with the charge applied across the entire horizontal vector... The individual used it for rapid large scale purification, by catching the right, left and middle flows down the plate...

  This strikes me as a novel method for purification/separation that could be accomplished with say,  electrolytess, glass plate, fiberglass cloth and 200 volts D.C., without the vac distillation etc...

 Any thoughts?

Infinite Radiant Light - THKRA
05-22-02 19:55
No 312790
      How exactly does that work?  Bookmark   

Is this like chromatography assisted by electric current or something?
(Distinctive Doe)
05-22-02 20:10
No 312799
      Not YET  Bookmark   

Not applicable to large scales.
They are struggling to get up to gram scale seps.

Those who give up essential liberties for temporary safety deserve neither liberty nor safety
(Hive Bee)
05-22-02 20:24
No 312810
      Large Scale?  Bookmark   

 so foxy, what's the problem with this in large scale?

Infinite Radiant Light - THKRA
(Distinctive Doe)
05-22-02 20:40
No 312817
      temerature  Bookmark   

your applying a huge voltage, which requires very good cooling or you cook the stuff.  Thats why they normally do electrophoresis in a capilary tube, easy to keep cool.

Those who give up essential liberties for temporary safety deserve neither liberty nor safety
(Hive Addict)
05-23-02 02:38
No 313046
      LSD  Bookmark   

Hmm might work for 100mg shitty acid. Make an gel plate (agar ?) ad the 100mg and aply power from the neontube (with a diode :)
(Hive Bee)
05-23-02 22:33
No 313465
      well...  Bookmark   

Not to beat this to death, but it seems that the problem is that the substances to separate ( and what we are interested in) have weak charges, so the separation is not efficient...

  Does anyone think that might work??

  What would happen if you irradiated the area with laser light tuned to the orbital wavelength of what you were interested in???  Similar to the atomic vapor isotope separation???  Then you would have a selective charge on your molecule, that should, afaik, make it more mobile across the voltage differential?

Infinite Radiant Light - THKRA
(Distinctive Doe)
05-24-02 01:16
No 313521
      capilary EP  Bookmark   

New capilary electrophoresis techniques have gotten separations of over a million theoretical plates.

Those who give up essential liberties for temporary safety deserve neither liberty nor safety
03-17-03 15:18
No 418186
      electrophorisis  Bookmark   

The migration is a function of molecular charge,
impressed voltage,
and media.

You can use 200 volts and watch it migrate,
or 24 volts and come back next week.(Exaggeration.)

A piece of blotter paper, between two glass plates,
with the overhanging ends immersed into the electrode

Hmm:  meth FB has almost zero charge: ephedrine does not have much.


Just put your 5 grams in the middle, and come back next week to collect your 2.3478 grams of pure shitola??

(Distinctive Doe)
03-17-03 20:23
No 418316
      Any free amine has lots of charge and should...  Bookmark   

Any free amine has lots of charge and should easily bee able to bee separated with electrophoresis, at very small scales.
(Hive Adickt)
03-18-03 11:23
No 418697
      fun  Bookmark   

īThe idea is fun, but I don't think it has any practical use. With 'standard' chromatography (dry colum ect.) you can seperate almost any thing in a few houers in the 10-100g scale.
03-18-03 15:14
No 418803
      A 'freebase' amine doesn't carry a charge.  Bookmark   

A 'freebase' amine doesn't carry a charge. If you want an amine to move in the electric field, you have to protonate it by adjusting the pH. smile