Chemikaze (HyperLab Bee)
07-15-02 05:57
No 332817
      Serotonin/Creatin-Sulfate???  Bookmark   

Bees,do you have any ideas on separation of serotonin and creatin(Glycine, N-(aminoiminomethyl)-N-methyl).
May be a bee with Merck could help?blush.
I guess Creatin is very soluble in waterlaugh...hmm well serotonin should be toolaugh?

When I die bury me upside down so the world can kiss my ass.
07-15-02 22:24
No 333138
      Serotonin-creatinine-sulfate?  Bookmark   

Are you sure you don't have a serotonin-creatinine-sulfate complex?

Creatinine is soluble in 12 parts water, slightly soluble in ethanol, practically insoluble in acetone, ether, chloroform.

Maybe basify the complex with ammonium hydroxide solution and extract with chloroform or methylene chloride?
(HyperLab Bee)
07-15-02 23:43
No 333163
      Okey Dokey  Bookmark   

Hmm,i guess it IS a complex,but is it creatinine or creatin because they are absoulutely different substances,or may be you mistyped Lilienthal?
SWIH are you reading this,is it creatine or creatinine you`ve got?

When I die bury me upside down so the world can kiss my ass.
(HyperLab Bee)
07-16-02 00:27
No 333181
      Yeah  Bookmark   

well, i have serotonine + creatine. Not creatinine.

So, what we need - solubility of cretine and serotonine in some different liquids. If Lilienthal "creatinine" creatine :), i need to know solubility of serotonine in acetone, chloroform and ether.

Tongue-tied and twisted just an earth-bound misfit, I
07-16-02 07:54
No 333275
      creatine & creatinine  Bookmark   

Under neutral or basic conditions there is an equilibrium between creatine and creatinine, while under acidic conditions there's only creatinine.

Under basic conditions the carboxy group should be deprotonated, making the molecule extremely hydrophilic compared to serotonine freebase. You can't use a stronger base than NH4OH because that would degrade the serotonin.


Molecule: creatinine ("NC(=N)N(C)CC(=O)O")

Creatinine is the cyclized form:

Molecule: creatinine ("C1C(=O)NC(=N)N1C")

(HyperLab Bee)
07-16-02 11:59
No 333320
      Hmmm  Bookmark   

It's a beautiful idea. Tnx, we try it.

Tongue-tied and twisted just an earth-bound misfit, I