(Hive Bee)
03-19-03 15:43
No 419065
      Articles on ergot cultivation
(Rated as: excellent)

It has been suggested that some of these articles might be useful for some people. They'll stay online a couple of weeks for those who want them.

Lloydia;1962;25;37-45;Pacifici et al;Production of Lysergic Acid Derivatives in Submerged Culture.  I. Fermentation Studies. (

Lloydia;1962;25;161-73;Pacifici et al;Production of Lysergic Acid Derivatives in Submerged Culture.  II. Strain Selection and Screening. (

Lloydia;1963;26;174-91;Gröger & Tyler;Alkaloid Production by Claviceps paspali in Submerged Culture. (

Adv. Appl. Microbiol.;1969;11;211-44;W.J. Kelleher;Ergot Alkaloid Fermentations. (
03-19-03 18:42
No 419111
      thats so much!  Bookmark   

Thanks that just what I was looking for. tongue

The bus came by&I got on,thats when it all began.-GD
03-19-03 23:16
No 419173
      ... which leads us to paspalic acid  Bookmark   

Bhankzzz you :)

According to one of those papers - and I quote -

"(Pacifici et al., 1962; Gröger and Tylet, 1936). Kobel
et al. (1964) then described an isolate of C. paspali which
produced high yields of delta-8,9 lysergic acid in submerged
culture. This product is readily converted to delta-9,10
lysergic acid by heating with dilute aqueous alkali"

the delta-8,9 lysergic acid must be paspalic acid
(we all know the conversion which has been posted on this
forum and which can also be found at

There are 3 different Delta-8,9's there:
Agroclavine, Elymoclavine and Molliclavine...
Molliclavine looks suspicious., C. paspali submerged cultures have ergine,
isoergine and lysergic acid N-1-hydroxyethylamide (Arcamone
et al., 1960) while sclerotia from Australia contain up to
0.005% alkaloids composed of ergine and ergonovine along
with chanoclavine and two unidentified ergoline alkaloids
(Groger et al., 1961). Elymoclavine (Kobel et al., 1964)
and agroclavine (Brar et al.,1968) have also been recorded.

there is something about Flieger M., Sedmera P., Havlíček V., Cvak L. Stuchlík J.: 10-OH cis and 10-OH trans paspalic acid amide - new alkaloid of Claviceps paspali.

Anybee got Journal of Natural products, 56 [6] 810-814 (1993)??

Now, SWIM is still only interested of paspalic acid and
wants to know how only (~95%) it can be produced

Or is everything extracted out from C. paspali regarded
as paspalic acid???

(Hive Bee)
03-20-03 00:14
No 419183
      This might also help
(Rated as: excellent)


US2056360 (
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US2809920 (
US2835675 (
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US2936266 (
GB883329 (
US3038840 (
US3060104 (
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Well I deserve a big excellent for this.

If there are more beautiful patents on the subject, please post them. Thanks. smile
(Hive Bee)
03-20-03 03:08
No 419230
      Thank You Bottleneck!!!  Bookmark   

You're the MAN! These will help so much in my fermentation research and experiments!
All you Bio-Bees out there: let's take the info Bottleneck and others have provided here and run with it. Setting up a fermenter isn't that hard, and once we find a good Lysergic producing strain, we can pass it around and share it. Growing a fungus in a culture medium is much easier than synthing some some chemicals together. Let's DO IT!
(Hive Addict)
03-20-03 03:24
No 419235
      The articles are gone  Bookmark   

Can someone that has downloaded them maybe also host them?

Accept No Imitations, There Can Only Bee One;
(Hive Bee)
03-20-03 04:52
No 419280
      excellent posts  Bookmark

excellent posts!
03-21-03 04:34
No 419658
      Yes man yes!  Bookmark   

Damn flipper now thats what we've been needing.Lets get this shit out an in the open.Lets break this shit down.The "their gone"faces are going for 17,500 a GRAM.Hows that grab ya!Grabs me by my balls!I'm sorry fellas I wont bee able to work with these rediculous prices.I feel like a gump!tongue

The bus came by&I got on,thats when it all began.-GD
03-21-03 05:07
      am I reading that right?????
(Rated as: insignificant)
(Hive Bee)
03-21-03 13:50
No 419786
      Paspalic acid  Bookmark   

Here is that article on the isolation of paspalic acid from certain races of Claviceps paspali.

Helvetica Chimica Acta;1964;47;1052-64;Kobel et al;6-Methyl-d8,9-ergolen-8-carbonsäure, ein neues Ergolinderivat aus Kulturen eines Stammes von Claviceps paspali Stevens et Hall. (