Bubbleplate (Hive Bee)
04-30-03 22:33
No 430459
      five-fold increase in total alkaloid production  Bookmark   

An article in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY describes a technique to increase alkaloid yield in Claviceps culture by adding Perfluorocarbons to the media. Perflurocarbons are being investigated and used for, among other things, synthetic blood.
If someone has access to and could post this article, it would be greatly appreciated!
Title:   Application of oxygen vectors to Claviceps purpurea cultivation
Author/s:   M Menge, J Mukherjee, T Scheper
ISSN   01757598    

Abstract:  The application of a two-phase fermentation system for the production of ergot peptide alkaloids by Claviceps purpurea is described. Perfluorocarbons (PFC) are used as oxygen vectors in Claviceps fermentation for the first time. In shake-flask cultivations, the inclusion of PFC in the medium brings about a five-fold increase in the total alkaloid production and a six-fold increase in the pharmaceutically important component, ergotamine.
(Chief Bee)
05-05-03 09:34
No 431486
      Application of oxygen vectors 2 Claviceps purpurea  Bookmark   

Full Paper: Appl. Microbiol. Biotechnol. 55, 411-416 (2001) (../rhodium/pdf /claviceps.purpurea.oxygen.vectors.pdf) DOI:10.1007/s002530100592
(Hive Bee)
07-07-03 18:13
No 445249
      Yes, another article also showed  Bookmark   

minimal or no increase using oxygen carriers.
Pacifici ("Production of Lysergic Acid Derivatives in Submerged Culture") found that the addition of 50 mg. L-Tryptophan & 1 gram Acetamide per liter of media increased alkaloid concentration 500% vs. no supplement.
(Hive Bee)
07-07-03 21:35
No 445292
      Bubbleplate> Are you sure it was ...  Bookmark   

Bubbleplate> Are you sure it was L-tryptophan that caused the increased production? As far as i remember it was the dl- version. I don't have the article here, so i can't be sure of course.

Size doesn't matter, it's the sound you make when hitting the ground after smoking it, that matters!
(Hive Bee)
07-08-03 01:13
No 445366
      I don't have the link to the article  Bookmark   

but I'm looking at a hard copy in my hands and it says:
"50 mg. L-tryptophan + 1 g. Acetamide".
Also, throughout the article, it states that "l-tryptophan" was used.
When I ordered Tryptophan, I never though to specify the type, and when it arrived, it was L-Tryptophan. Maybe that is the common type?